The Effects of Temperature and Relative Humidity on the Viability of the Coronavirus

Abstract

The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log₁₀) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of >95%). The better stability of SARS coronavirus at low temperature and low humidity environment may facilitate its transmission in community in subtropical area (such as Hong Kong) during the spring and in air-conditioned environments. It may also explain why some Asian countries in tropical area (such as Malaysia, Indonesia or Thailand) with high temperature and high relative humidity environment did not have major community outbreaks of SARS.

1. Introduction

Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [1–3]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8].
We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [9, 10]. Another study showed that it was inactivated by ultraviolet light, alkaline (), or acidic () conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [12–14] but they only survive a few hours after drying [13, 14]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [15–17]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity.

2. Material and Methods

2.1. Virus Strain and Cell Line

The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO₂ and were used for growing stock virus and for titration of viral infectivity [1, 2].

2.2. Preparation of Stock Virus

Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use.

2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID₅₀)

96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10⁻¹ to 10⁸. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID₅₀ was determined according to Reed and the Muench method [19].

2.4. Effect of Drying, Heat, and Relative Humidity

Ten microlitre of maintenance medium containing 10⁷ TCID₅₀ per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying.
The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated.

2.5. Infectivity Assay

The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10⁻¹ to 10⁸ was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID₅₀ was determined according to the Reed and Muench method [19].

3. Results

Ten microlitre of 10⁷ TCID₅₀ per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only  loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments.

Residual virus infectivity at 22–25°C with relative humidity 40–50% (starting titre 10⁵/10 μL) and at 33°C or 38°C with relative humidity >95%.

High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5  loss of titre for each time point up to 24 hr (0.38~3.38 ) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)).

Infectivity of SARS Coronavirus (10⁵/10 μL) to different temperatures at (a) >95% relative humidity, (b) >80–89%.

4. Discussion

Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [20, 22]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting.
In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community.
Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for.
Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed.
In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [15–17]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [16, 17]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [10, 15–17, 24, 25]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009.

Table 1

WHO SARS report—based on data as of the 31st December 2003.

Areas Total Medan age Deaths Case fatality Ratio (%) No. of imported Cases (%) No. of HCW (%) First case Last case China 5327 NKn 349 7 NA 1002 (19) Nov-02 Jun-03 Hong Kong 1755 40 299 17 NA 386 (22) Feb-03 May-03 Taiwan 346 42 37 11 21 (6) 68 (20) Feb-03 Jun-03 Singapore 238 35 33 14 8 (3) 97 (41) Feb-03 May-03 Viet Nam 63 43 5 8 1 (2) 36 (57) Feb-03 Apr-03 Indonesia 2 56 0 0 2 (100) 0 (0) Apr-03 Apr-03 Malaysia 5 30 2 40 5 (100) 0 (0) Mar-03 Apr-03 Thailand 9 42 2 22 9 (100) 1 (11) Mar-03 May-03 Philippines 14 41 2 14 7 (50) 4 (29) Feb-03 May-03 Total 8096 774 9.6 142 1706 (21)

Table 2

A summary of the meteorological data of 2005 in average weather conditions*.

Month Average sunlight (hours) Temperature Discomfort from heat and humidity Relative humidity Min Max am pm (a) Kuala Lumpur, Malaysia Jan 6 22 32 High 97 60 Feb 7 22 33 High 97 60 March 7 23 33 High 97 58 April 6 23 33 High 97 63 May 6 23 33 High 97 66 June 7 22 33 High 96 63 July 7 23 32 High 95 63 Aug 6 23 32 High 96 62 Sept 6 23 32 High 96 64 Oct 5 23 32 High 96 65 Nov 5 23 32 High 97 66 Dec 5 22 32 High 97 61 (b) Jakarta, Indonesia Jan 5 23 29 High 95 75 Feb 5 23 29 High 95 75 March 6 23 30 High 94 73 April 7 24 31 High 94 71 May 7 24 31 High 94 69 June 7 23 31 High 93 67 July 7 23 31 High 92 64 Aug 8 23 31 High 90 61 Sept 8 23 31 High 90 62 Oct 7 23 31 High 90 64 Nov 6 23 30 High 92 68 Dec 5 23 29 High 92 71 (c) Bangkok, Thailand Jan 9 20 32 High 91 53 Feb 8 22 33 High 92 55 March 9 24 34 High 92 56 April 8 25 35 Extreme 90 58 May 8 25 34 Extreme 91 64 June 6 24 33 Extreme 90 67 July 5 24 32 High 91 66 Aug 5 24 32 High 92 66 Sept 5 24 32 High 94 70 Oct 6 24 31 High 93 70 Nov 8 22 31 High 92 65 Dec 9 20 31 High 91 56 (d) Singapore Jan 5 23 30 High 82 78 Feb 7 23 31 High 77 71 March 6 24 31 High 76 70 April 6 24 31 High 77 74 May 6 24 32 Extreme 79 73 June 6 24 31 High 79 73 July 6 24 31 High 79 72 Aug 6 24 31 High 78 72 Sept 5 24 31 High 79 72 Oct 5 23 31 High 78 72 Nov 5 23 31 High 79 75 Dec 4 23 31 High 82 78 (e) Hong Kong Jan 5 13 18 — 77 66 Feb 4 13 17 — 82 73 March 3 16 19 — 84 74 April 4 19 24 Medium 87 77 May 5 23 28 Medium 87 78 June 5 26 29 High 86 77 July 8 26 31 High 87 77 Aug 6 26 31 High 87 77 Sept 6 25 29 High 83 72 Oct 7 23 27 Medium 75 63 Nov 7 18 23 Moderate 73 60 Dec 6 15 20 — 74 63

*Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results).

Conflict of Interests

The authors declare that there is no conflict of interest.

References

1. J. S. M. Peiris, S. T. Lai, L. L. M. Poon et al., “Coronavirus as a possible cause of severe acute respiratory syndrome,” The Lancet, vol. 361, no. 9366, pp. 1319–1325, 2003.View at: Publisher Site | Google Scholar
2. T. G. Ksiazek, D. Erdman, C. S. Goldsmith et al., “A novel coronavirus associated with severe acute respiratory syndrome,” New England Journal of Medicine, vol. 348, no. 20, pp. 1953–1966, 2003.View at: Publisher Site | Google Scholar
3. C. Drosten, S. Günther, W. Preiser et al., “Identification of a novel coronavirus in patients with severe acute respiratory syndrome,” New England Journal of Medicine, vol. 348, no. 20, pp. 1967–1976, 2003.View at: Publisher Site | Google Scholar
4. C. A. Donnelly, A. C. Ghani, G. M. Leung et al., “Epidemiological determinants of spread of causal agent of severe acute respiratory syndrome in Hong Kong,” The Lancet, vol. 361, no. 9371, pp. 1761–1766, 2003.View at: Publisher Site | Google Scholar
5. T. F. Booth, B. Kournikakis, N. Bastien et al., “Detection of airborne severe acute respiratory syndrome (SARS) coronavirus and environmental contamination in SARS outbreak units,” Journal of Infectious Diseases, vol. 191, no. 9, pp. 1472–1477, 2005.View at: Publisher Site | Google Scholar
6. Department of Health, “Outbreak of sever acute respiratory syndrome (SARS) at Amoy Gardens, Kowloon Bay, Hong Kong main findings of the investigation,” 2003.View at: Google Scholar
7. J. S. M. Peiris, C. M. Chu, V. C. C. Cheng et al., “Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study,” The Lancet, vol. 361, no. 9371, pp. 1767–1772, 2003.View at: Publisher Site | Google Scholar
8. I. T. S. Yu, Y. Li, T. W. Wong et al., “Evidence of airborne transmission of the severe acute respiratory syndrome virus,” New England Journal of Medicine, vol. 350, no. 17, pp. 1731–1739, 2004.View at: Publisher Site | Google Scholar
9. WHO Report, “First data on stability and resistance of SARS coronavirus compiled by members of WHO laboratory network,” http://www.who.int/csr/sars/survival_2003_05_04/en/#.View at: Google Scholar
10. M. Y. Lai, P. K. Cheng, and W. W. Lim, “Survival of severe acute respiratory syndrome coronavirus,” Clinical Infectious Diseases, vol. 41, no. 7, pp. e67–e71, 2005.View at: Google Scholar
11. M. E. R. Darnell, K. Subbarao, S. M. Feinstone, and D. R. Taylor, “Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV,” Journal of Virological Methods, vol. 121, no. 1, pp. 85–91, 2004.View at: Publisher Site | Google Scholar
12. M. K. Ijaz, A. H. Brunner, and S. A. Sattar, “Survival characteristics of airborne human coronavirus 229E,” Journal of General Virology, vol. 66, no. 12, pp. 2743–2748, 1985.View at: Google Scholar
13. J. Sizun, M. W. N. Yu, and P. J. Talbot, “Survival of human coronaviruses 229E and OC43 in suspension and after drying on surfaces: a possible source of hospital-acquired infections,” Journal of Hospital Infection, vol. 46, no. 1, pp. 55–60, 2000.View at: Publisher Site | Google Scholar
14. H. F. Rabenau, J. Cinatl, B. Morgenstern, G. Bauer, W. Preiser, and H. W. Doerr, “Stability and inactivation of SARS coronavirus,” Medical Microbiology and Immunology, vol. 194, no. 1-2, pp. 1–6, 2005.View at: Publisher Site | Google Scholar
15. J. Tan, L. Mu, J. Huang, S. Yu, B. Chen, and J. Yin, “An initial investigation of the association between the SARS outbreak and weather: with the view of the environmental temperature and its variation,” Journal of Epidemiology and Community Health, vol. 59, no. 3, pp. 186–192, 2005.View at: Publisher Site | Google Scholar
16. J. Yuan, H. Yun, W. Lan et al., “A climatologic investigation of the SARS-CoV outbreak in Beijing, China,” American Journal of Infection Control, vol. 34, no. 4, pp. 234–236, 2006.View at: Publisher Site | Google Scholar
17. Q. C. Cai, J. Lu, Q. F. Xu et al., “Influence of meteorological factors and air pollution on the outbreak of severe acute respiratory syndrome,” Public Health, vol. 121, no. 4, pp. 258–265, 2007.View at: Publisher Site | Google Scholar
18. L. M. Casanova, S. Jeon, W. A. Rutala, D. J. Weber, and M. D. Sobsey, “Effects of air temperature and relative humidity on coronavirus survival on surfaces,” Applied and Environmental Microbiology, vol. 76, no. 9, pp. 2712–2717, 2010.View at: Publisher Site | Google Scholar
19. L. J. Reed and H. Muench, “A simple method of estimating fifty per cent endpoints,” American Journal of Epidemiology, vol. 27, no. 3, pp. 493–497, 1938.View at: Google Scholar
20. C. B. Hall, R. G. Douglas, and J. M. Geiman, “Possible transmission by fomites of respiratory syncytial virus,” Journal of Infectious Diseases, vol. 141, no. 1, pp. 98–102, 1980.View at: Google Scholar
21. E. C. Pirtle and G. W. Beran, “Virus survival in the environment,” OIE Revue Scientifique et Technique, vol. 10, no. 3, pp. 733–748, 1991.View at: Google Scholar
22. M. T. Brady, J. Evans, and J. Cuartas, “Survival and disinfection of parainfluenza viruses on environmental surfaces,” American Journal of Infection Control, vol. 18, no. 1, pp. 18–23, 1990.View at: Google Scholar
23. W. H. Seto, D. Tsang, R. W. H. Yung et al., “Effectiveness of precautions against droplets and contact in prevention of nosocomial transmission of severe acute respiratory syndrome (SARS),” The Lancet, vol. 361, no. 9368, pp. 1519–1520, 2003.View at: Publisher Site | Google Scholar
24. K. Lin, D. Y. K. Fong, B. Zhu, and J. Karlberg, “Environmental factors on the SARS epidemic: air temperature, passage of time and multiplicative effect of hospital infection,” Epidemiology and Infection, vol. 134, no. 2, pp. 223–230, 2006.View at: Publisher Site | Google Scholar
25. V. S. F. Chan, K. Y. K. Chan, Y. Chen et al., “Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection,” Nature Genetics, vol. 38, no. 1, pp. 38–46, 2006.View at: Publisher Site | Google Scholar